Hybrid lipid nanoparticles derived from human mesenchymal stem cell extracellular vesicles by microfluidic sonication for collagen I mRNA delivery to human tendon progenitor stem cells
Rubén Pareja Tello, Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Jouni Hirvonen, Gonçalo Barreto, Nicola Mafulli, Giovanna Della Porta, Hélder A. Santos
Abstract
efficacy studies on TSPCs showed a 2-fold increase in procollagen type I carboxy-terminal peptide production comparable with the effect caused by LNPs. Therefore, our work provides an alternative production method for MSC-EV-derived hybrid NPs and supports their potential use as drug delivery systems for tendon regeneration.
Topics & Concepts
Mesenchymal stem cellCell biologyStem cellChemistrySonicationTendonProgenitor cellVesicleMicrovesiclesBiophysicsBiochemistryBiologyAnatomyMembranemicroRNAGeneChromatographyTendon Structure and Treatment