AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses
Yumi Jang, Fred Bunz
Abstract
assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).
Topics & Concepts
GenomeRecombinant DNAModular designComputational biologyComputer sciencePlasmidBiologyVector (molecular biology)Adenovirus genomeProtocol (science)GeneVirologyAdenoviridaeGeneticsProgramming languagePathologyMedicineAlternative medicineVirus-based gene therapy researchViral Infectious Diseases and Gene Expression in InsectsViral gastroenteritis research and epidemiology