Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering
Yannian Gou, Yanran Huang, Wenping Luo, Yanan Li, Piao Zhao, Jiamin Zhong, Xiangyu Dong, Meichun Guo, Aohua Li, Ailing Hao, Guozhi Zhao, Yonghui Wang, Yi Zhu, Hui Zhang, Yunhan Shi, William Wagstaff, Hue H. Luu, Lewis L. Shi, Russell R. Reid, Tong‐Chuan He, Jiaming Fan
Abstract
, whereas iMAD and iCAL exhibited highest osteogenic capability in BMP9-induced ectopic osteogenesis and critical-sized calvarial defect repair. Transcriptomic analysis revealed that, while each MSC line regulated a distinct set of target genes upon BMP9 stimulation, all MSC lines underwent osteogenic differentiation by regulating osteogenesis-related signaling including Wnt, TGF-β, PI3K/AKT, MAPK, Hippo and JAK-STAT pathways. Collectively, our results demonstrate that adipose-derived MSCs represent optimal progenitor sources for cell-based bone tissue engineering.