Facile Access to Far‐Red Fluorescent Probes with Through‐Space Charge‐Transfer Effects for In Vivo Two‐Photon Microscopy of the Mouse Cerebrovascular System
Yingjuan Hong, Weihang Geng, Tian Zhang, Guangshuai Gong, Chongyang Li, Canze Zheng, Feng Liu, Jun Qian, Ming Chen, Ben Zhong Tang
Abstract
Much effort has been devoted to the generation of fluorescent probes by synthetic approaches. In this study, we developed a facile strategy to construct far-red fluorescent probes based on through-space charge transfer within complexes of acceptors and donors and their "twist+twist" interactions. Owing to their rare two-photon excitation property, the complexes could be used for in vivo imaging of the mouse cerebrovascular system.
Topics & Concepts
FluorescenceTwo-photon excitation microscopyIn vivoExcitationBiophysicsFluorescence microscopeCharge (physics)PhotonMicroscopyChemistryMaterials scienceNanotechnologyPhotochemistryPhysicsOpticsBiologyQuantum mechanicsBiotechnologyLuminescence and Fluorescent MaterialsPhotochromic and Fluorescence ChemistryNanoplatforms for cancer theranostics