Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells
Johan Chin-Kang Tay, Junjian Wang, Zhicheng Du, Yu Yang Ng, Qiang Li, Yuefang Ren, Chang Zhang, Jianqing Zhu, Xuehu Xu, Shu Wang
Abstract
anti-tumor activity of NKG2D CAR-T cells. Interestingly, the expression of T cell exhaustion markers TIGIT, TIM3, and LAG3 was reduced with extended manufacturing. To enhance the safety profile of the NKG2D CAR-T cells, we incorporated a full-length CD20 transgene in tandem with the CAR construct and demonstrated that autologous NK cells could mediate efficient antibody-dependent cell-mediated cytotoxicity to remove these CAR-T cells. Collectively, our study illustrates a protocol that generates large numbers of efficacious NKG2D CAR-T cells suitable for multiple rounds of infusions.
Topics & Concepts
Transposable elementNKG2DK562 cellsAntigenChimeric antigen receptorBiologyComputer scienceCell biologyCytotoxic T cellImmunologyGeneticsCell cultureGeneImmune systemT cellGenomeIn vitroCAR-T cell therapy researchImmune Cell Function and InteractionViral Infectious Diseases and Gene Expression in Insects