Rapid Detection of Staphylococcus aureus in Milk and Pork via Immunomagnetic Separation and Recombinase Polymerase Amplification
Runan Cheng, Lei Li, Sihui Zhen, Honglei Liu, Zhouhui Wu, Yu Wang, Zhen Wang
Abstract
Our study established IMBs-RPA method, which simplified the steps of bacteria separation, shortened the detection time, and realized the convenient detection of S. aureus in milk and pork samples. IMBs-RPA method was also suitable for the detection of other pathogens, providing a new method for food safety monitoring and a favorable basis for rapid and early diagnosis of diseases.
Topics & Concepts
Staphylococcus aureusImmunomagnetic separationRecombinase Polymerase AmplificationPolymerase chain reactionMicrobiologyReal-time polymerase chain reactionBacteriaBiologyGeneGeneticsBiosensors and Analytical DetectionIdentification and Quantification in FoodSalmonella and Campylobacter epidemiology