Epithelial uptake leads to fungal killing in vivo and is aberrant in COPD-derived epithelial cells
Margherita Bertuzzi, Gareth Howell, Darren D. Thomson, Rachael Fortune-Grant, Anna Möslinger, Patrick Dancer, Norman van Rhijn, Natasha Motsi, Alice Codling, Elaine Bignell
Abstract
Hundreds of spores of Aspergillus fumigatus (Af) are inhaled daily by human beings, representing a constant, possibly fatal, threat to respiratory health. The small size of Af spores suggests that interactions with alveolar epithelial cells (AECs) are frequent; thus, we hypothesized that spore uptake by AECs is important for driving fungal killing and susceptibility to Aspergillus -related disease. Using single-cell approaches to measure spore uptake and its outcomes in vivo , we demonstrate that Af spores are internalized and killed by AECs during whole-animal infection. Moreover, comparative analysis of primary human AECs from healthy and chronic obstructive pulmonary disease (COPD) donors revealed significant alterations in the uptake and killing of spores in COPD-derived AECs. We conclude that AECs contribute to the killing of Af spores and that dysregulation of curative AEC responses in COPD may represent a driver of Aspergillus -related diseases.