Encapsulated DNase improving the killing efficiency of antibiotics in staphylococcal biofilms
Chenhui Liu, Yu Zhao, Wanqi Su, Jingshan Chai, Lina Xu, Jingjing Cao, Yang Liu
Abstract
We developed a polymer-encapsulated DNase, n(DNase), which can efficiently accumulate in biofilm and expose the DNase to cleave the eDNA of the biofilm. CLSM and crystal violet staining results demonstrated effective biofilm disintegration (92.2%) when treated with n(DNase). This work demonstrated a general approach for coating matrix-dispersion enzymes to achieve biofilm disintegration and provided a promising strategy for treating biofilm-associated infections.
Topics & Concepts
BiofilmAntibioticsMicrobiologyMaterials scienceBacteriaBiologyGeneticsBacterial biofilms and quorum sensingAntimicrobial Peptides and ActivitiesBiosensors and Analytical Detection