SpheroidChip: Patterned Agarose Microwell Compartments Harboring HepG2 Spheroids are Compatible with Genotoxicity Testing
Christy Chao, Le P. Ngo, Bevin P. Engelward
Abstract
-induced DNA damage. In summary, we utilized an agarose microarray to condense what had required an entire 96-well plate into a single well, enabling analysis techniques that were cumbersome or impossible under conditions of a single spheroid per well of a 96-well plate.
Topics & Concepts
SpheroidAgaroseComet assayGenotoxicityDNA damageAgarose gel electrophoresisChemistryDNACell biologyMolecular biologyBiophysicsBiologyIn vitroToxicityBiochemistryOrganic chemistry3D Printing in Biomedical ResearchPluripotent Stem Cells ResearchAnimal testing and alternatives