Purified recombinant enzymes efficiently hydrolyze conjugated urinary (poly)phenol metabolites
Jacob Lessard‐Lord, Pier-Luc Plante, Yves Desjardins
Abstract
achieved similar efficiency for only 28 conjugated MPMs after 6 h of hydrolysis. When comparing the concentration of unconjugated MPMs released after enzymatic hydrolysis, recombinant enzymes were more or as effective for almost every MPM. These results demonstrate that accurate quantification of MPMs in urine can be quickly achieved using purified recombinant enzymes and represent an affordable alternative to the use of conjugated analytical standards, improving access to the analysis of the metabolism of (poly)phenols by the gut microbiota.
Topics & Concepts
HydrolysisChemistryChromatographyHelix pomatiaConjugated systemEnzymatic hydrolysisRecombinant DNAEnzymeArylsulfataseBiochemistryOrganic chemistryBiologyEcologySnailGenePolymerMetabolomics and Mass Spectrometry StudiesGut microbiota and healthNutritional Studies and Diet