Sequestration of Proteins in Stress Granules Relies on the In-Cell but Not the <i>In Vitro</i> Folding Stability
Nirnay Samanta, Sara S. Ribeiro, Mailin Becker, Émeline Laborie, Roland Pollak, Štěpán Timr, Fabio Sterpone, Simon Ebbinghaus
Abstract
experiments however suggest that the increased flexibility of the unfolded state constitutes only a minor driving force to associate with the dynamic biomolecular network of the condensate. Specific protein-protein interactions in the cytoplasm in comparison to SGs determine the partitioning of folding states between the respective phases during HS.
Topics & Concepts
ChemistryProtein foldingFolding (DSP implementation)OrganelleBiophysicsCytoplasmProtein stabilityProtein aggregationUnfolded protein responseIn vitroSOD1Intrinsically disordered proteinsFunction (biology)Cell biologyBiochemistrySuperoxide dismutaseEndoplasmic reticulumEnzymeBiologyEngineeringElectrical engineeringRNA Research and SplicingHeat shock proteins researchEndoplasmic Reticulum Stress and Disease