A fluorescent probe for specifically measuring the overall thioredoxin and glutaredoxin reducing activity in bacterial cells
Xin Zuo, Ying Zhao, Jintao Zhao, Yanfang Ouyang, Wenjun Qian, Yinmei Hou, Chong Yu, Xiaoyuan Ren, Lili Zou, Jianguo Fang, Jun Lu
Abstract
Both bacterial thioredoxin and glutaredoxin systems can reduce TRFS-green selectively, which confers TRFS-green to be a remarkable probe to detect the dominant disulfide reductase activity with a slow reaction rate in bacteria, e. g. E. coli Grx2&3.
Topics & Concepts
GlutaredoxinThioredoxin reductaseThioredoxinChemistryFluorescenceBacteriaFerredoxin-thioredoxin reductaseBiochemistryCombinatorial chemistryEnzymeBiologyPhysicsGeneticsQuantum mechanicsRedox biology and oxidative stressSulfur Compounds in BiologyMetal-Catalyzed Oxygenation Mechanisms