Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis
Zhongyang Lv, Jie Han, Jiawei Li, Hu Guo, Yuxiang Fei, Ziying Sun, Jian Dong, Maochun Wang, Chunmei Fan, Weitong Li, Ya Xie, Wei Sun, Jiaqi Chen, Yuan Liu, Fufei Chen, Zizheng Liu, Anlong Liu, Rui Wu, Xingquan Xu, Wenjin Yan, Qing Jiang, Shiro Ikegawa, Xiao Chen, Dongquan Shi
Abstract
BACKGROUND: Osteoarthritis (OA) is the most common degenerative joint disease primarily characterized by cartilage destruction. The aim of this study was to investigate the role, molecular characteristics and potential therapeutic target of chondrocyte ferroptosis in the pathogenesis of OA. METHODS: ). FINDINGS: mice. INTERPRETATION: TRPV1 activation protects chondrocytes from ferroptosis and ameliorates OA progression by upregulating GPX4. FUNDING: National Key R&D Program of China (2018YFC1105904), Key Program of NSFC (81730067), National Science Foundation of China (81772335, 81941009, 81802196), Natural Science Foundation of Jiangsu Province, China (BK20180127), Jiangsu Provincial Key Medical Talent Foundation, Six Talent Peaks Project of Jiangsu Province (WSW-079).