Microfluidic organ-on-a-chip model of the outer blood–retinal barrier with clinically relevant read-outs for tissue permeability and vascular structure
Yusuf B. Arık, Wesley Buijsman, Joshua Loessberg-Zahl, Carlos Cuartas-Vélez, Colin Veenstra, Sander Logtenberg, Anne M. Grobbink, Piet Bergveld, Giuliana Gagliardi, Anneke I. den Hollander, Nienke Bosschaart, Albert van den Berg, Robert Passier, Andries D. van der Meer
Abstract
), a dose dependent increase in barrier permeability was observed by using a dynamic assay for fluorescence tracing, analogous to the clinically used fluorescence angiography. This organ-on-a-chip of the oBRB will allow future studies of complex disease mechanisms and treatments for visual disorders using clinically relevant endpoints in vitro.
Topics & Concepts
Blood–retinal barrierOrgan-on-a-chipRetinalVascular permeabilityTissue fluidPermeability (electromagnetism)Microfluidic chipMicrofluidicsPathologyMedicineBiologyBiomedical engineeringOphthalmologyMaterials scienceNanotechnologyBiochemistryEndocrinologyDiabetic retinopathyMembraneDiabetes mellitusRetinal Diseases and Treatments3D Printing in Biomedical ResearchRetinal Development and Disorders