Microbial-derived metabolites induce actin cytoskeletal rearrangement and protect blood-brain barrier function
Emily G. Knox, María R. Aburto, Carmen Tessier, Jatin Nagpal, Gerard Clarke, Caitríona M. O’Driscoll, John F. Cryan
Abstract
BBB model treated with physiologically relevant concentrations of butyrate and propionate with and without lipopolysaccharide (LPS) to examine the effects of SCFAs on the actin cytoskeleton and tight junction protein structure. Both SCFAs induced distinct alterations to filamentous actin directionality. SCFAs also increased tight junction protein spikes and protected from LPS-induced tight-junction mis-localization, improved BBB integrity, and modulated mitochondrial network dynamics. These findings identify the actin cytoskeletal dynamics as another target further illuminating how SCFAs can influence BBB physiology.
Topics & Concepts
Tight junctionCytoskeletonBlood–brain barrierCell biologyButyrateActinActin cytoskeletonBarrier functionBiologyPropionateChemistryBiophysicsBiochemistryNeuroscienceCentral nervous systemCellFermentationBarrier Structure and Function StudiesGut microbiota and healthAdvanced Neuroimaging Techniques and Applications