Temocillin Resistance in the Enterobacter cloacae Complex Is Conferred by a Single Point Mutation in BaeS, Leading to Overexpression of the AcrD Efflux Pump
François Guérin, François Gravey, Sophie Reissier, Malo Penven, Charlotte Michaux, Simon Le Hello, Vincent Cattoir
Abstract
conferred a significant increase (from 8- to 16-fold) of the temocillin MIC. Altogether, we have shown that temocillin resistance in the ECC can result from a single BaeS alteration, likely resulting in the permanent phosphorylation of BaeR and leading to AcrD overexpression and temocillin resistance through enhanced active efflux.
Topics & Concepts
Enterobacter cloacaeEffluxBiologyPlasmidPoint mutationEscherichia coliMicrobiologyMutagenesisEnterobacterWild typeMolecular biologyEnterobacteriaceaeGeneticsMutationMutantGeneAntibiotic Resistance in BacteriaPlant Pathogenic Bacteria StudiesBacterial Genetics and Biotechnology