Tungsten disulfide Quantum Dots Based Disposable Paper Based Lab on GenoChip for Specific Meningitis DNA Detection
Riya Gupta, Manila Ozhukil Valappil, Arunkumar Sakthivel, Ashish Mathur, Chandra Shekhar Pundir, Murugavel Kathiresan, Jagriti Narang, Subbiah Alwarappan
Abstract
Tungsten disulfide quantum dots (WS 2 QDs) embedded lab on genochip based analytical device (PAD) was developed for the specific DNA detection of Meningitis, a life threatening disease. This paper-based lab on genochip was designed by grafting the two electrodes onto the surface of paper using conductive ink. The WS 2 QDs were characterized by X-ray diffraction technique, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Following this, specific oligonucleotide sequence of DNA (capture probe) was immobilized over the surface of WS 2 QDs/PAD electrode. The ssDNA/WS 2 QDs/PAD device was characterized electrochemically by cyclic voltammetry. The device was employed for detection of target DNA (by hybridization) by employing methylene blue (MB) as an indicator. The biosensor exhibited a wide linear response in the range 1 nM–100 μM towards the target DNA. The limit of detection (LOD) of target DNA of the proposed device is 1 nM. The advantageous features of this device than the existing biosensors include cheaper analysis cost, tiny sample requirements and stability.