Thermodynamics of adsorption of alcohol dehydrogenase on the gold nanoparticle surface: a model based analysis <i>versus</i> direct measurement
Akriti Mishra, Kamini Mishra, Dipayan Bose, Abhijit Chakrabarti, Puspendu K. Das
Abstract
. However, the adsorption is modestly endothermic, accompanied by a large increase in entropy. Stated differently, GNP-ADH corona formation is entropically driven. This is perhaps due to the tremendous disruption of the water structure at the negatively charged interface upon the arrival of the protein within the bonding distance to it. We believe that the SHLS technique is highly sensitive and reliable, at very low concentrations of both nanoparticles and proteins, for the quantitative estimation of the thermodynamic parameters of nanoparticle-protein corona formation, where many other techniques may fall short.
Topics & Concepts
ChemistryAdsorptionAlcohol dehydrogenaseGibbs free energyLangmuir adsorption modelLangmuirDynamic light scatteringColloidal goldAqueous solutionNanoparticleThermodynamicsBinding constantGibbs isothermPhysical chemistryMaterials scienceBinding siteAlcoholOrganic chemistryNanotechnologyPhysicsBiochemistryGold and Silver Nanoparticles Synthesis and ApplicationsProtein Interaction Studies and Fluorescence AnalysisNanoparticle-Based Drug Delivery