Driving forces of the complex formation between highly charged disordered proteins
Aritra Chowdhury, Alessandro Borgia, Souradeep Ghosh, Andrea Sottini, Soumik Mitra, Rohan Eapen, Madeleine B. Borgia, Tianjin Yang, Nicola Galvanetto, Miloš T. Ivanović, Paweł Łukijańczuk, Ruijing Zhu, Daniel Nettels, Arindam Kundagrami, Benjamin Schuler
Abstract
Highly disordered complexes between oppositely charged intrinsically disordered proteins present a new paradigm of biomolecular interactions. Here, we investigate the driving forces of such interactions for the example of the highly positively charged linker histone H1 and its highly negatively charged chaperone, prothymosin α (ProTα). Temperature-dependent single-molecule Förster resonance energy transfer (FRET) experiments and isothermal titration calorimetry reveal ProTα-H1 binding to be enthalpically unfavorable, and salt-dependent affinity measurements suggest counterion release entropy to be an important thermodynamic driving force. Using single-molecule FRET, we also identify ternary complexes between ProTα and H1 in addition to the heterodimer at equilibrium and show how they contribute to the thermodynamics observed in ensemble experiments. Finally, we explain the observed thermodynamics quantitatively with a mean-field polyelectrolyte theory that treats counterion release explicitly. ProTα-H1 complex formation resembles the interactions between synthetic polyelectrolytes, and the underlying principles are likely to be of broad relevance for interactions between charged biomolecules in general.