Coronavirus Endoribonuclease Ensures Efficient Viral Replication and Prevents Protein Kinase R Activation
Ye Zhao, Lu Sun, Ye Zhao, Delan Feng, Jinlong Cheng, Guozhong Zhang
Abstract
Coronaviruses can emerge from animal reservoirs into naive host species to cause pandemic respiratory and gastrointestinal diseases with significant mortality in humans and domestic animals. Infectious bronchitis virus (IBV), a γ-coronavirus, infects respiratory, renal and reproductive systems, causing millions of dollars in lost revenue worldwide annually. Mutating the viral endoribonuclease resulted in an attenuated virus and prevented protein kinase R activation. Therefore, EndoU activity is a virulence factor in IBV infections, thus providing an approach for generating live-attenuated vaccine candidates for emerging coronaviruses.
Topics & Concepts
BiologyVirologyViral replicationEndoribonucleaseCoronavirusReplication (statistics)KinaseViral entryVirusCell biologyCoronavirus disease 2019 (COVID-19)GeneticsRNAGeneInfectious disease (medical specialty)DiseaseMedicinePathologyRNase PSARS-CoV-2 and COVID-19 ResearchEndoplasmic Reticulum Stress and DiseaseAnimal Virus Infections Studies