TargetDBP+: Enhancing the Performance of Identifying DNA-Binding Proteins via Weighted Convolutional Features
Jun Hu, Liang Rao, Yiheng Zhu, Guijun Zhang, Dong‐Jun Yu
Abstract
Protein–DNA interactions exist ubiquitously and play important roles in the life cycles of living cells. The accurate identification of DNA-binding proteins (DBPs) is one of the key steps to understand the mechanisms of protein–DNA interactions. Although many DBP identification methods have been proposed, the current performance is still unsatisfactory. In this study, a new method, called TargetDBP+, is developed to further enhance the performance of identifying DBPs. In TargetDBP+, five convolutional features are first extracted from five feature sources, i.e., amino acid one-hot matrix (AAOHM), position-specific scoring matrix (PSSM), predicted secondary structure probability matrix (PSSPM), predicted solvent accessibility probability matrix (PSAPM), and predicted probabilities of DNA-binding sites (PPDBSs); second, the five features are weightedly and serially combined using the weights of all of the elements learned by the differential evolution algorithm; and finally, the DBP identification model of TargetDBP+ is trained using the support vector machine (SVM) algorithm. To evaluate the developed TargetDBP+ and compare it with other existing methods, a new gold-standard benchmark data set, called UniSwiss, is constructed, which consists of 4881 DBPs and 4881 non-DBPs extracted from the UniprotKB/Swiss-Prot database. Experimental results demonstrate that TargetDBP+ can obtain an accuracy of 85.83% and precision of 88.45% covering 82.41% of all DBP data on the independent validation subset of UniSwiss, with the MCC value (0.718) being significantly higher than those of other state-of-the-art control methods. The web server of TargetDBP+ is accessible at http://csbio.njust.edu.cn/bioinf/targetdbpplus/; the UniSwiss data set and stand-alone program of TargetDBP+ are accessible at https://github.com/jun-csbio/TargetDBPplus.