NrtR Mediated Regulation of H1-T6SS in Pseudomonas aeruginosa
Xinxin Zhang, Liwen Yin, Qi Liu, Dan Wang, Congjuan Xu, Xiaolei Pan, Fang Bai, Zhihui Cheng, Weihui Wu, Yongxin Jin
Abstract
and pcnA to repress the expression of the two operons, therefore controlling the NAD biosynthesis. We have previously reported that NrtR controls T3SS expression via the cAMP/Vfr pathway in P. aeruginosa. However, the global regulatory function and direct binding targets of the NrtR remain elusive in P. aeruginosa. This study reveals novel direct regulatory targets of the NrtR in P. aeruginosa, elucidating the molecular mechanism of NrtR-mediated regulation of H1-T6SS.
Topics & Concepts
OperonChromatin immunoprecipitationPseudomonas aeruginosaBiologyRegulation of gene expressionTranscriptional regulationPromoterMutantNAD+ kinaseRegulonType VI secretion systemElectrophoretic mobility shift assayCell biologyGeneGene expressionGeneticsBacteriaBiochemistryEnzymeVirulenceVibrio bacteria research studiesBacterial biofilms and quorum sensingBacterial Genetics and Biotechnology