Characterizing the Contributions of Various Clostridium perfringens Enterotoxin Properties to <i>In Vivo</i> and <i>In Vitro</i> Permeability Effects
Archana Shrestha, Mauricio A. Navarro, Juliann Beingesser, Aníbal G. Armién, Francisco A. Uzal, Bruce A. McClane
Abstract
permeability effects. Absorption of binding-capable, noncytotoxic rCPE variants from the intestines did not correlate with general intestinal permeability alterations, suggesting that CPE binding can induce its own uptake. These findings highlight the importance of binding and, especially, cytotoxicity for CPE absorption during enterotoxemia and may assist development of permeability-altering rCPE variants for translational purposes.
Topics & Concepts
EnterotoxinClostridium perfringensIn vivoIn vitroMicrobiologyCytotoxicityChemistryBiologyEscherichia coliBacteriaBiochemistryBiotechnologyGeneGeneticsClostridium difficile and Clostridium perfringens researchGut microbiota and healthProbiotics and Fermented Foods