Green synthesis, characterization, antimicrobial and anti-cancer activity of silver nanoparticles using Aristolochia bracteolate plant leaf extract: experimental and theoretical calculations
Periyan Durairaju, Ghazala Kaukab Raja, G. Venkatesh, Chandramohan Govindasamy, Ahmed S. El Newehy, S. Haseena, P. Vennila, S. Balasubramaniyan
Abstract
Silver nanoparticles were synthesized using Aristolochia bracteolate plant extracts. The nanoparticles were subsequently studied using various techniques such as UV-Vis, FTIR, EDS, XRD, SEM, and HR-TEM analysis. MTT assays have been utilized to determine the cytotoxicity of green synthesized silver nanoparticles at concentrations ranging from 2.5 to 15 μg. The Ab-AgNPs exhibited effective cytotoxic activity against MCF-7. Anticancer activity studies revealed that Ab-AgNps produced comparable good results for standard cancer drugs. Ab-AgNPs are used for evaluating their antibacterial effectiveness against pathogenic gram-positive and gram-negative bacteria. Aristolochic acid (AA1) and Aristolochic acid (AA2) are the major phytoconstituents in the Aristolochia bracteolate. The current study reports theoretical investigations of aristolochic acids (AA1 and AA2) as well as silver nanocages aristolochic acids using density functional theory. Furthermore, the silver-doped with Aristolochic acids (AA1 and AA2) was subjected and examined their of breast cancer activity using molecular docking analysis. KEY WORDS: Aristolochia bracteolate, Silver nanoparticles, Transmission electron microscopy, Docking, Anti cancer active Bull. Chem. Soc. Ethiop. 2025, 39(2), 271-286. DOI: https://dx.doi.org/10.4314/bcse.v39i2.7