Uric acid detection <i>via</i> dual-mode mechanism with copper-coordinated nitrogen-doped carbon dots as peroxidase mimics
Yousef A. Bin Jardan, Mohamed M. El‐Wekil, Mohamed R. Elmasry, Al‐Montaser Bellah H. Ali
Abstract
an inner-filter effect, enabling fluorometric detection. Additionally, for colorimetric detection, the pink compound was quantified by measuring absorbance at 510 nm. The detection strategy utilized the peroxidase-mimetic activity of Cu@N-CDs, which was further enhanced by the presence of Cu. Under optimized conditions, the fluorometric method demonstrated a linear detection range of 0.01-700 μM, while the colorimetric method showed a range of 0.07-700 μM for uric acid. The developed approach proved highly effective in detecting uric acid in human blood serum and urine samples, yielding accurate results with acceptable recovery rates. This dual-mode detection method offers a reliable, sensitive, and cost-effective tool for monitoring uric acid levels, marking a significant advancement in clinical diagnostics and personalized healthcare.