Proteomic and metabolomic profiling combined with <i>in vitro</i> studies reveal the antiproliferative mechanism of silver nanoparticles in MDA-MB-231 breast carcinoma cells
J. Grace Nirmala, Kimaya Meher, Manu Lopus
Abstract
assembled, purified tubulin and immunofluorescence imaging of cellular microtubules. Proteomic and metabolomic analyses revealed the downregulation of lipid metabolism to be a synergistic contributor to cell death. Taken together, we report a novel antiproliferative mechanism of action of T-AgNPs that involves tubulin disruption and the downregulation of lipid metabolism.
Topics & Concepts
Clonogenic assayMechanism of actionFlow cytometryCell biologyCytotoxicityChemistryCancer cellDownregulation and upregulationMicrotubuleIn vitroBiophysicsMetabolomicsProgrammed cell deathBiochemistryBiologyApoptosisMolecular biologyCancerGeneChromatographyGeneticsNanoparticle-Based Drug DeliveryComputational Drug Discovery MethodsNanoparticles: synthesis and applications