Caproicibacterium amylolyticum gen. nov., sp. nov., a novel member of the family Oscillospiraceae isolated from pit clay used for making Chinese strong aroma-type liquor
Yang Gu, Xiaojun Zhu, Feng Lin, Caihong Shen, Yong Li, Ao L, Wenlai Fan, Cong Ren, Yan Xu
Abstract
An anaerobic, Gram-stain-positive, rod-shaped, motile and spore-forming bacterium, designated strain LBM18003 T , was isolated from pit clay used for making Chinese strong aroma-type liquor. Growth occurred at 20–40 °C (optimum, 30–37 °C), pH 4.5–9.5 (optimum, pH 6.5–7.0) and in the presence of 0.0–1.0 % (w/v) sodium chloride (optimum, 0 %). The predominant fatty acids were C 16:0 , C 14:0 , C 14:0 DMA and C 16:0 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and nine unidentified glycolipids. Phylogenetic analysis revealed that strain LBM18003 T is a novel member of the family Oscillospiraceae . The 16S rRNA gene sequence similarities of strain LBM18003 T to its two most closely related species were less than 94.5 % for distinguishing genera, i.e. closely related to Caproiciproducens galactitolivorans JCM 30532 T (94.1 %) and Caproicibacter fermentans DSM 107079 T (93.2 %). The genome size of strain LBM18003 T was 2 996 201 bp and its DNA G+C content was 48.48 mol%. Strain LBM18003 T exhibited 67.8 and 68.1% pairwise-determined whole-genome average nucleotide identity values to Caproiciproducens galactitolivorans JCM 30532 T and Caproicibacter fermentans DSM 107079 T , respectively; and showed 62.2 and 61.0 % the average amino acid identity values to Caproiciproducens galactitolivorans JCM 30532 T and Caproicibacter fermentans DSM 107079 T , respectively; and demonstrated 46.1 and 41.5 % conserved genes to Caproiciproducens galactitolivorans JCM 30532 T and Caproicibacter fermentans DSM 107079 T , respectively. The comparisons of 16S rRNA gene and genome sequences confirmed that strain LBM18003 T represented a novel genus of the family Oscillospiraceae . Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain LBM18003 T represents a novel species of a novel genus of the family Oscillospiraceae , for which the name Caproicibacterium amylolyticum gen. nov., sp. nov. is proposed. The type strain is LBM18003 T (=GDMCC 1.1626 T =JCM 33783 T ).