Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites
Laura Atkinson, Jack C. D. Lee, Alexander Lennon, Divya Shah, Nathaniel Storey, Sofia Morfopoulou, Kathryn Harris, Judy Breuer, Julianne R. Brown
Abstract
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.
Topics & Concepts
MetagenomicsProtocol (science)Cerebrospinal fluidNucleic acidBiologyComputational biologyMedicinePathologyGeneticsGeneAlternative medicineCytomegalovirus and herpesvirus researchMycobacterium research and diagnosisHIV Research and Treatment