Thermodynamic consequences of Tyr to Trp mutations in the cation–π-mediated binding of trimethyllysine by the HP1 chromodomain
Mackenzie W. Krone, Katherine I. Albanese, Gage O. Leighton, Cyndi Qixin He, Ga Young Lee, Marc Garcia‐Borràs, Alex J. Guseman, David C. Williams, K. N. Houk, Eric M. Brustad, Marcey L. Waters
Abstract
In this work, we experimentally validate that tryptophan provides the strongest cation–π binding interaction among aromatic amino acids and also lend insight into the importance of residue identity in trimethyllysine recognition by reader proteins.
Topics & Concepts
ChemistryChromodomainMutationStereochemistryBiochemistryHelicaseRNAGeneProtein Structure and DynamicsMass Spectrometry Techniques and ApplicationsSpectroscopy and Quantum Chemical Studies