Rapid Hypothesis Testing in Candida albicans Clinical Isolates Using a Cloning-Free, Modular, and Recyclable System for CRISPR-Cas9 Mediated Mutant and Revertant Construction
Junyan Liu, Amanda K. Vogel, Jian Miao, Jennifer Carnahan, David J. Lowes, Jeffrey M. Rybak, Brian M. Peters
Abstract
Recently, phenotypic heterogeneity in Candida albicans isolates has been recognized as an underappreciated factor contributing to gene diversification and broadly impacts strain-to-strain antifungal resistance, fitness, and pathogenicity. We have designed a cloning-free genetic system for rapid gene deletion and reversion in C. albicans clinical isolates that interlaces established recyclable genetic systems with CRISPR-Cas9 technology.
Topics & Concepts
CRISPRMutantCandida albicansCloning (programming)BiologyModular designComputational biologyCas9GeneticsGeneComputer scienceProgramming languageAntifungal resistance and susceptibilityCRISPR and Genetic EngineeringPlant tissue culture and regeneration