Layer-by-Layer Assembly of Polycations and Polyanions for the Sensitive Detection of Endotoxin
Biyas Posha, Haritha Kuttoth, N. Sandhyarani
Abstract
Bacterial endotoxin detection is an essential safety requisite in biomedical, food, and pharmaceutical industries. Endotoxin in a sufficient concentration on entering the human bloodstream causes detrimental effects such as septic shock, which can lead to death. Hence, the sensitive and selective detection of endotoxin also known as lipopolysaccharide (LPS) is of paramount importance. Herein, a layer-by-layer (LBL) assembly of gold-chitosan nanocomposite (CGNC)-poly(acrylic acid) (PAA)-polymyxin B (PmB) on gold (Au) electrode is employed for the sensitive and selective detection of endotoxin. The surface electric charge studies using dynamic contact mode electrostatic force microscopy (DC-EFM) revealed the successful formation of each layer on the Au electrode. The polycationic PmB is a specific bioreceptor of LPS, which binds with high affinity to the anionic groups of the carbohydrate portions of LPS molecules and facilitates the selective electrochemical detection. This surface modification method presented a sensitive and selective detection of endotoxin down to the attogram level.