A label-free and ultrasensitive electrochemical biosensor for oral cancer overexpressed 1 gene <i>via</i> exonuclease III-assisted target recycling and dual enzyme-assisted signal amplification strategies
Dongyou Zhang, Yali Wang, Xiaoyu Jin, Qi Xiao, Shan Huang
Abstract
exonuclease III-assisted target recycling and dual enzyme-assisted signal amplification strategies. The electrochemical impedance value was linearly related to the logarithmic concentration of ORAOV1 gene in the range from 0.05 fM to 20 pM, and the detection limit of ORAOV1 gene was low to 0.019 fM. This biosensor was used to detect ORAOV1 gene in complicated human saliva samples with satisfactory results.
Topics & Concepts
ExonucleaseChemistryDNAExonuclease IIIHeminOligonucleotideGuanineMolecular biologyBiochemistryDNA polymeraseEnzymeGeneBiologyNucleotideEscherichia coliHemeAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA and protein synthesis mechanisms