A single aromatic residue in sgp130Fc/olamkicept allows the discrimination between interleukin-6 and interleukin-11 trans-signaling
Juliane Lokau, Yvonne Garbers, Joachim Grötzinger, Christoph Garbers
Abstract
are different between the two cytokines. Modification of an aromatic cluster around Q113 of gp130 within these interfaces allows the discrimination between IL-6 and IL-11 trans-signaling. Using recombinant sgp130Fc variants, we demonstrate that these differences can indeed be exploited to generate a truly selective IL-6 trans-signaling inhibitor. Our data highlight how the selectivity of a clinically relevant designer protein can be further improved.
Topics & Concepts
Glycoprotein 130CytokineSignal transductionReceptorInterleukinChemistryCell biologyComputational biologyInterleukin 6BiologyBiochemistryImmunologyCytokine Signaling Pathways and InteractionsToxin Mechanisms and ImmunotoxinsMonoclonal and Polyclonal Antibodies Research