Visual monitoring of the lysosomal pH changes during autophagy with a red-emission fluorescent probe
Xiaodong Wang, Li Fan, Yubin Wang, Caihong Zhang, Wenting Liang, Shaomin Shuang, Chuan Dong
Abstract
value is determined as 4.96, and the linear response with pH changes from 4.50-5.70, which is favorable for lysosomal pH imaging. We also confirm that RML diffuses selectively into lysosomes using confocal fluorescence microscopy. Using RML, we have successfully visualized autophagy by monitoring the lysosomal pH changes.
Topics & Concepts
FluorescenceAutophagyBiophysicsMaterials scienceLysosomeNanotechnologyChemistryBiochemistryBiologyOpticsApoptosisEnzymePhysicsAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolismAdenosine and Purinergic Signaling