The phosphatase-like activity of zirconium oxide nanoparticles and their application in near-infrared intracellular imaging
Xilu Hu, Ting Huang, Hong Liao, Lianzhe Hu, Min Wang
Abstract
In this study, the phosphatase mimetic activity of zirconium oxide nanoparticles (ZrO2 NPs) has been demonstrated. They can effectively catalyze the dephosphorylation of a series of commercial fluorogenic and chromogenic substrates of natural phosphatases. Compared with natural phosphatases, ZrO2 NPs possess several advantages such as low cost, facile preparation procedures, and high stability in a broader pH range or at high temperatures. In addition, the activity of ZrO2 NPs toward some important biomolecules was investigated. The ZrO2 NPs can catalyze the dephosphorylation of ATP and o-phospho-l-tyrosine, but they cannot react with DNA strands. These data are important for the further bio-related applications of ZrO2 NPs. Finally, the potential application of ZrO2 NPs in intracellular imaging is also demonstrated by using a near-infrared fluorescent substrate of alkaline phosphatase.