Screening for probiotic attributes of lactic acid bacteria isolated from human milk and evaluation of their anti-diabetic potentials
Manoj Kumar, Sukumaran Karthika, Nair Anjitha, Perumal Varalakshmi, Balasubramaniem Ashokkumar
Abstract
Lactic acid bacteria (LAB) have gained much attention recently due to their probiotic attributes with anti-ACE and anti-glycation potentials targeting management of diabetes. This study was designed to isolate LAB from human milk and to evaluate their potential for inhibitory activities on ACE and AGEs formation using <i>in vitro</i> assay models. Among 12 LAB isolates, many strains exhibited strong probiotic attributes including tolerance to low pH, high salt, gastric, and intestinal juices with higher cell adhesion. Further, cell-free extracts of LAB tested for <i>in vitro</i> anti-ACE and anti-AGEs activities evidenced <i>Lacticaseibacillus casei</i> MKU1, <i>Lacticaseibacillus paracasei</i> MKU2, <i>Lactiplantibacillus pentosus</i> MKU3, <i>L. paracasei</i> MKU7, <i>L. delbrueckii</i> GERU3 and <i>Lactobacillus delbrueckii</i> MKU10 as strong ACE and AGEs inhibitors. Subsequently, Principal Component Analysis showed a positive correlation with anti-ACE (<i>p</i> ≤ .01) and anti-glycation (<i>p</i> ≤ .05) activity of LAB. Overall, this study suggests that six <i>Lactobacillus</i> isolates of human milk with superior probiotic attributes looks promising as potential probiotic strains.