<i>N</i>-Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy
Kilian Roßmann, Kerem Can Akkaya, Pascal Poc, Corentin Charbonnier, Jenny Eichhorst, Hannes Gonschior, Abha Valavalkar, Nicolas Wendler, Thorben Cordes, Benjamin Dietzek‐Ivanšić, Ben Jones, Martin Lehmann, Johannes Broichhagen
Abstract
brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.