Entrectinib approval by EMA reinforces options for ROS1 and tumour agnostic NTRK targeted cancer therapies
Elena Ardini, Salvatore Siena
Abstract
On 28 May 2020, the Committee for Medicinal Products for Human Use (CHMP) of the European Medicines Agency (EMA) recommended the granting of a conditional marketing authorisation for entrectinib (Rozlytrek), for the treatment of patients whose solid tumours have a neurotrophic tyrosine receptor kinase (NTRK) gene fusion or for patients with ROS1 fusion-positive advanced non-small cell lung cancer (NSCLC).1.This site uses cookies to offer you a better browsing experience. Find out more on how we use cookies and how you can change your settings. Available: https://www.ema.europa.eu/en/medicines/human/summaries-opinion/rozlytrekGoogle Scholar Based on the full indication, entrectinib represents a new therapeutic option for the treatment of adult and paediatric patients 12 years of age and older, with solid tumours NTRK fusion-positive, who have a disease that is locally advanced, metastatic or where surgical resection is likely to result in severe morbidity, and who have not received a prior NTRK inhibitor, who have no satisfactory treatment options. In addition, entrectinib is indicated as monotherapy for the treatment of adult patients with ROS1 fusion-positive, advanced NSCLC not previously treated with ROS1 inhibitors. The present CHMP recommendation is based on the analysis of combined results of four clinical studies, the pivotal phase II STARTRK-2, the ALKA-372–001 and the STARTRK-1 phase I trials, and the phase I/II STARTRK-NG paediatric study.2.CHMP recommends EU approval of Roche’s Rozlytrek for people with NTRKfusion-positive solid tumours and for people with ROS1-positive, advanced nonsmall cell lung cancer. Available: https://www.roche.com/dam/jcr:17344668-eab7-4e12-9098-0d940cdc16eb/en/29052020-roche-mediarelease-chmp-recommends-eu-approval-of-r.pdfGoogle Scholar Overall in these studies, the efficacy of entrectinib was observed in patients with NTRK fusion-positive locally advanced or metastatic tumours, with an objective response rate (ORR) of 63.5% and a median duration of response (DoR) of 12.9 months.2.CHMP recommends EU approval of Roche’s Rozlytrek for people with NTRKfusion-positive solid tumours and for people with ROS1-positive, advanced nonsmall cell lung cancer. Available: https://www.roche.com/dam/jcr:17344668-eab7-4e12-9098-0d940cdc16eb/en/29052020-roche-mediarelease-chmp-recommends-eu-approval-of-r.pdfGoogle Scholar The clinical benefit reported across several different NTRK fusion-positive tumour types is in our opinion a very compelling evidence and supports the tissue-agnostic indication approval for entrectinib. This approval would represent the second case, after larotrectinib, of an EMA granted approval based on a common driver molecular alteration across different tumour types rather than on tumour histology. In ROS1 fusion-positive advanced NSCLC patients enrolled in the trials, entrectinib achieved ORR in 73.4% of cases with a median DoR of 16.5 months (14.6–28.6 months).2.CHMP recommends EU approval of Roche’s Rozlytrek for people with NTRKfusion-positive solid tumours and for people with ROS1-positive, advanced nonsmall cell lung cancer. Available: https://www.roche.com/dam/jcr:17344668-eab7-4e12-9098-0d940cdc16eb/en/29052020-roche-mediarelease-chmp-recommends-eu-approval-of-r.pdfGoogle Scholar Importantly, ORR of 67.1% was observed in the subset of patients with central nervous system (CNS) metastases at baseline, consistent with the efficient brain penetration of entrectinib clearly demonstrated during its preclinical characterisation3.Ardini E. Menichincheri M. Banfi P. et al.Entrectinib, a pan-Trk, ROS1, and ALK inhibitor with activity in multiple molecularly defined cancer indications.http://www.ncbi.nlm.nih.gov/pubmed/26939704Mol Cancer Ther. 2016; 15: 628-639doi:10.1158/1535-7163.MCT-15-0758Google Scholar and then confirmed in the clinical settings.2.CHMP recommends EU approval of Roche’s Rozlytrek for people with NTRKfusion-positive solid tumours and for people with ROS1-positive, advanced nonsmall cell lung cancer. Available: https://www.roche.com/dam/jcr:17344668-eab7-4e12-9098-0d940cdc16eb/en/29052020-roche-mediarelease-chmp-recommends-eu-approval-of-r.pdfGoogle Scholar The most common adverse reactions were fatigue, constipation, dysgeusia, oedema, dizziness, diarrhoea, nausea, nervous system disorders (dysesthesia), shortness of breath (dyspnoea), anaemia, increased weight, increased blood creatinine, pain, cognitive disorders, vomiting, cough and fever.1.This site uses cookies to offer you a better browsing experience. Find out more on how we use cookies and how you can change your settings. Available: https://www.ema.europa.eu/en/medicines/human/summaries-opinion/rozlytrekGoogle Scholar In light of these clinical results and in the context of affirmation of precision oncology, entrectinib approval by EMA reinforces options for ROS1 and tumour-agnostic NTRK targeted cancer therapies. The recommendation of the CHMP for conditional marketing authorisation is the most recent achievement in the all recent history of this compound that in 2017 had been granted Priority Medicines designation by the EMA and Breakthrough Therapy Designation by Food and Drug Administration (FDA) for the treatment of NTRK fusion-positive, locally advanced or metastatic solid tumours. Entrectinib is already marketed in the USA where on 15 August 2019 FDA has issued an accelerated, tissue-agnostic approval to the drug to target solid tumour types bearing NTRK fusions and for the treatment of metastatic ROS1 fusion-positive NSCLC and in Japan where it was approved in June 2019 for the treatment of patients with NTRK fusion-positive tumours and in February 2020 for the treatment of patients with ROS1 fusion-positive NSCLC.4.Drilon A. Siena S. Ou S.-H.I. et al.Safety and antitumor activity of the multitargeted pan-Trk, ROS1, and ALK inhibitor Entrectinib: combined results from two phase I trials (ALKA-372-001 and STARTRK-1).http://www.ncbi.nlm.nih.gov/pubmed/28183697Cancer Discov. 2017; 7: 400-409doi:10.1158/2159-8290.CD-16-1237Google Scholar, 5.Doebele R.C. Drilon A. Paz-Ares L. et al.Entrectinib in patients with advanced or metastatic NTRK fusion-positive solid tumours: integrated analysis of three phase 1-2 trials.http://www.ncbi.nlm.nih.gov/pubmed/31838007Lancet Oncol. 2020; 21: 271-282doi:10.1016/S1470-2045(19)30691-6Google Scholar, 6.Drilon A. Siena S. Dziadziuszko R. et al.Entrectinib in ROS1 fusion-positive non-small-cell lung cancer: integrated analysis of three phase 1-2 trials.http://www.ncbi.nlm.nih.gov/pubmed/31838015Lancet Oncol. 2020; 21: 261-270doi:10.1016/S1470-2045(19)30690-4Google Scholar, 7.AVDesai, GMBrodeur, JFoster, et al. STARTRK-NG: a phase 1/1b study of Entrectinib in children and adolescents with advanced solid tumors and primary CNS tumors, with or without Trk, ROS1, or ALK fusions. Cancer Res;77.Google Scholar The registration of entrectinib for NTRK fusion-positive tumours represents the pharmaceutical conclusion of a scientific history lasting for more than three decades (figure 1). In 1983 Mariano Barbacid, working in the lab of Stuart Aaronson, identified in a colorectal cancer specimen an inversion within chromosome 1 resulting in a fusion oncogene that was named TRK (tropomyosin receptor kinase). This fusion oncogene was not found in subsequent analyses and it was considered an oddity.8.Barbacid M. On the right Trk: from oncogene discovery to cancer therapeutics.http://www.ncbi.nlm.nih.gov/pubmed/32223936Ann Oncol. 2019; 30 Suppl 8: viii3-4doi:10.1093/annonc/mdz290Google Scholar The full length neurotrophin receptor TRK was identified a few years later, and the chromosome 1 inversion was not further investigated.9.Martin-Zanca D. Hughes S.H. Barbacid M. A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences.http://www.ncbi.nlm.nih.gov/pubmed/2869410Nature. 1986; 319: 743-748doi:10.1038/319743a0Google Scholar Probably at that time, nobody could have imagined that the clinical relevance of that finding would have been fully exploited many years later when thank to the availability of new sequencing technology but also to a certain dose of serendipity we stepped into during our work of preclinical and clinical investigators. More than two decades later, at the Italian pharmaceutical company Nerviano Medical Sciences, as part of a standard drug discovery process, the preclinical characterisation of a novel drug was going to be completed. The molecule, now known as entrectinib (former NMS-E628), discovered and optimised to be a potent, brain penetrant ROS1- and ALK-inhibitor was indeed found to be also active on TRK kinases, encoded by the NTRK genes.3.Ardini E. Menichincheri M. Banfi P. et al.Entrectinib, a pan-Trk, ROS1, and ALK inhibitor with activity in multiple molecularly defined cancer indications.http://www.ncbi.nlm.nih.gov/pubmed/26939704Mol Cancer Ther. 2016; 15: 628-639doi:10.1158/1535-7163.MCT-15-0758Google Scholar, 10.Menichincheri M. Ardini E. Magnaghi P. et al.Discovery of Entrectinib: a new 3-Aminoindazole as a potent anaplastic lymphoma kinase (ALK), c-ros oncogene 1 kinase (ROS1), and Pan-Tropomyosin receptor kinases (Pan-TRKs) inhibitor.http://www.ncbi.nlm.nih.gov/pubmed/27003761J Med Chem. 2016; 59: 3392-3408doi:10.1021/acs.jmedchem.6b00064Google Scholar This activity was considered since the very beginning an interesting opportunity for the development of the drug but it is fair to say that at that time the reports of NTRK fusions were still mostly anecdotal. The full exploitation of this opportunity became clear when the extensive cellular profiling of entrectinib revealed an exquisitely high antiproliferative activity of the drug in a colorectal cancer cell line that did not express either ALK or ROS1. The subsequent genetic characterisation demonstrated that this peculiar sensitivity was due to the presence of an inversion within chromosome 1 resulting in the generation of a NTRK fusion oncogene that was the driver for proliferation and survival of those cells. The very same chromosomal alteration found by Barbacid many years before in a surgically removed colorectal cancer specimen was indeed present in this cell line.11.Ardini E. Bosotti R. Borgia A.L. et al.The TPM3-NTRK1 rearrangement is a recurring event in colorectal carcinoma and is associated with tumor sensitivity to TrkA kinase inhibition.http://www.ncbi.nlm.nih.gov/pubmed/24962792Mol Oncol. 2014; 8: 1495-1507doi:10.1016/j.molonc.2014.06.001Google Scholar The valuable and productive collaboration between Nerviano Medical Science and Niguarda Cancer Center resulted in the setup in a record time of a validated screening method based on the use of immunohistochemistry (IHC), reverse transcriptase-polymerase chain reaction (RT-PCR) and in-situ hybridisation (ISH) that allowed to screen a number of colorectal cancer patients and to demonstrate that, even if with low incidence, NTRK rearrangements could be identified in a discrete subset of patients with colorectal cancer. This evidence represented the rationale for the inclusion of patients with NTRK fusion-positive tumours in the upcoming ALKA-372–001 phase I clinical trial of entrectinib.11.Ardini E. Bosotti R. Borgia A.L. et al.The TPM3-NTRK1 rearrangement is a recurring event in colorectal carcinoma and is associated with tumor sensitivity to TrkA kinase inhibition.http://www.ncbi.nlm.nih.gov/pubmed/24962792Mol Oncol. 2014; 8: 1495-1507doi:10.1016/j.molonc.2014.06.001Google Scholar The clinical benefit observed with a partial response achieved after 1 month of treatment in the first patient with NTRK fusion-positive colorectal cancer enrolled represented the first clinical proof of concept validation of NTRK fusions as targets for therapy in patients with colorectal cancer and spurred the search of such rearrangements in many additional tumour types.12.Sartore-Bianchi A. Ardini E. Bosotti R. et al.Sensitivity to Entrectinib Associated With a Novel LMNA-NTRK1 Gene Fusion in Metastatic Colorectal Cancer.http://www.ncbi.nlm.nih.gov/pubmed/26563355J Natl Cancer Inst. 2016; 108 (doi [Epub ahead of print 12 Nov 2015])doi:10.1093/jnci/djv306Google Scholar, 13.Amatu A. Sartore-Bianchi A. Bencardino K. et al.Tropomyosin receptor kinase (trk) biology and the role of NTRK gene fusions in cancer.Annals of Oncology. 2019; 30: viii5-15doi:10.1093/annonc/mdz383Abstract Full Text Full Text PDF PubMed Scopus (107) Google Scholar, 14.Drilon A. Trk inhibitors in Trk fusion-positive cancers.Annals of Oncology. 2019; 30: viii23-30doi:10.1093/annonc/mdz282Abstract Full Text Full Text PDF PubMed Scopus (138) Google Scholar Both Ignyta, a US biotech company that in 2013 acquired the rights for the development of entrectinib, and Roche that after the acquisition of Ignyta in 2017 developed the molecule up to the registration, have continued with wilfulness and determination the clinical exploration of entrectinib activity across many different NTRK fusion-positive tumour types. These joint efforts resulted, as already mentioned, in the tumour-agnostic approval of entrectinib and most importantly to high and durable therapeutic benefits for most of the treated patients.5.Doebele R.C. Drilon A. Paz-Ares L. et al.Entrectinib in patients with advanced or metastatic NTRK fusion-positive solid tumours: integrated analysis of three phase 1-2 trials.http://www.ncbi.nlm.nih.gov/pubmed/31838007Lancet Oncol. 2020; 21: 271-282doi:10.1016/S1470-2045(19)30691-6Google Scholar Compared to the development of the drug in NTRK fusion-positive tumours, the exploration of entrectinib efficacy in ROS1 fusion-positive NSCLC patients was quite straightforward. In the preclinical models entrectinib was shown to be an extremely potent ROS1 inhibitor and the direct comparison of activity in cells demonstrated its superiority with respect to crizotinib.3.Ardini E. Menichincheri M. Banfi P. et al.Entrectinib, a pan-Trk, ROS1, and ALK inhibitor with activity in multiple molecularly defined cancer indications.http://www.ncbi.nlm.nih.gov/pubmed/26939704Mol Cancer Ther. 2016; 15: 628-639doi:10.1158/1535-7163.MCT-15-0758Google Scholar In addition, as already mentioned, the molecule had been specifically optimised to penetrate the CNS and all the subsequent preclinical investigations demonstrated that entrectinib was indeed able to efficiently cross the blood brain barrier and to achieve efficacious exposure in the brain in all preclinical models tested.3.Ardini E. Menichincheri M. Banfi P. et al.Entrectinib, a pan-Trk, ROS1, and ALK inhibitor with activity in multiple molecularly defined cancer indications.http://www.ncbi.nlm.nih.gov/pubmed/26939704Mol Cancer Ther. 2016; 15: 628-639doi:10.1158/1535-7163.MCT-15-0758Google Scholar, 10.Menichincheri M. Ardini E. Magnaghi P. et al.Discovery of Entrectinib: a new 3-Aminoindazole as a potent anaplastic lymphoma kinase (ALK), c-ros oncogene 1 kinase (ROS1), and Pan-Tropomyosin receptor kinases (Pan-TRKs) inhibitor.http://www.ncbi.nlm.nih.gov/pubmed/27003761J Med Chem. 2016; 59: 3392-3408doi:10.1021/acs.jmedchem.6b00064Google Scholar This evidence supported the inclusion of patients with CNS involvement at baseline since the initial phase I study of the clinical development. The substantial intracranial activity of entrectinib confirmed in the clinical settings is particularly relevant for the treatment of patients with ROS1-positive NSCLC because of the high frequency of brain metastases at the diagnosis in this population and the suboptimal ability of crizotinib to penetrate the brain.6.Drilon A. Siena S. Dziadziuszko R. et al.Entrectinib in ROS1 fusion-positive non-small-cell lung cancer: integrated analysis of three phase 1-2 trials.http://www.ncbi.nlm.nih.gov/pubmed/31838015Lancet Oncol. 2020; 21: 261-270doi:10.1016/S1470-2045(19)30690-4Google Scholar The granting of marketing authorisation of entrectinib by EMA will represent for many European patients the availability of a new valuable therapeutic option. For the 1%–2% of patients with NSCLC bearing ROS1 fusions and especially for those with CNS involvement at diagnosis, this drug will represent a remarkable opportunity for achieving durable clinical benefit as foreseen in the plethora of NTRK fusion-positive tumours across different histologies. The application of the most appropriate approaches to optimise the selection of patients who can benefits from entrectinib treatment is crucial. The testing of ROS1 gene fusions in metastatic non-squamous NSCLC is considered mandatory in most European countries and based on European Society for Medical Oncology (ESMO) recommendation should be performed with ISH while IHC may be used to identify candidate tumours for confirmatory ISH testing.15.Planchard D. Popat S. Kerr K. et al.Metastatic non-small cell lung cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up.Annals of Oncology. 2018; 29: iv192-237doi:10.1093/annonc/mdy275Abstract Full Text Full Text PDF PubMed Scopus (1219) Google Scholar, 16.We've re-designed and re-organised our website so a few Pages may have moved. we've done our very, very best to set up highly-sophisticated page re-directs, but a few Pages – like the one you were hoping to see – may have slipped through the net. Available: https://www.esmo.org/Guidelines/Lung-and-Chest-Tumours/Metastatic-Non-Small-Cell-Lung-CancerGoogle Scholar The testing of NTRK fusions remains definitely more challenging mostly because of the broad patient population to be tested. Based on the recently issued ESMO recommendations, a conservative approach should be applied in order to identify any potential patients who can harbour these targetable genetic alterations and might benefit from a treatment with a NTRK inhibitor. Still, this approach will imply that different screening strategies should be applied to different patient populations.17.Marchiò C. Scaltriti M. Ladanyi M. et al.ESMO recommendations on the standard methods to detect NTRK fusions in daily practice and clinical research.http://www.ncbi.nlm.nih.gov/pubmed/31268127Ann Oncol. 2019; 30: 1417-1427doi:10.1093/annonc/mdz204Google Scholar For NTRK testing in histological tumour types where NTRK genes are frequently rearranged any chemiluminescence immunoassay (CLIA) validated method is applicable with ISH and nested RT-PCR being probably the most cost-effective ones. On the other hand, for the identification of NTRK fusions in an unselected population (histology-agnostic screening) the combination of next generation sequencing (NGS) and IHC is recommended. Depending on the health institution availability of the NGS targeted panel (DNA or RNA-based) the approach for testing could be different. NGS as initial screening with IHC used for confirmation of protein expression in positive cases would be ideal but, in case on unavailability of a targeted sequencing assay, IHC for initial screening followed by external sequencing for confirmation of any detected positivity is acceptable.17.Marchiò C. Scaltriti M. Ladanyi M. et al.ESMO recommendations on the standard methods to detect NTRK fusions in daily practice and clinical research.http://www.ncbi.nlm.nih.gov/pubmed/31268127Ann Oncol. 2019; 30: 1417-1427doi:10.1093/annonc/mdz204Google Scholar The EMA conditional approval of entrectinib further highlights the need to routinely test for NTRK fusions to broaden the therapeutic options available for patients. At this point, as part of a fruitful marketing strategy, we expect Roche, leveraging on its well-recognised expertise in diagnostics and in conjunction with its allied company Foundation Medicine, to make available a companion diagnostic that will help identify individuals with malignancies who may benefit from treatment with entrectinib. In an ideal world, all patients with cancer should have rapid access to all newly available anticancer drugs when approved. However, this is far from the reality and with the variations in economic standard across the EU, access to new cancer medicines differs significantly, especially in Eastern and South-Eastern European countries.18.Wilking N. Bucsics A. Kandolf Sekulovic L. et al.Achieving equal and timely access to innovative anticancer drugs in the European Union (EU): summary of a multidisciplinary CECOG-driven roundtable discussion with a focus on eastern and south-eastern EU countries.http://www.ncbi.nlm.nih.gov/pubmed/31798977ESMO Open. 2019; 4e000550doi:10.1136/esmoopen-2019-000550Google Scholar Access delays can be caused directly or indirectly by national or regional decision-making processes on reimbursement.18.Wilking N. Bucsics A. Kandolf Sekulovic L. et al.Achieving equal and timely access to innovative anticancer drugs in the European Union (EU): summary of a multidisciplinary CECOG-driven roundtable discussion with a focus on eastern and south-eastern EU countries.http://www.ncbi.nlm.nih.gov/pubmed/31798977ESMO Open. 2019; 4e000550doi:10.1136/esmoopen-2019-000550Google Scholar The two key aspects for those involved in reimbursement decisions are first the level of evidence required to decide and second pricing, which can be challenging for some innovative oncology compounds such as entrectinib. The ESMO has declared achieving equal access to cancer care as one of its major goals19.ESMO 2012 press release: equal access to cancer care is a medical and ethical imperative. Available: https://www.esmo.org/meetings/past-meetings/esmo-congress-2012/News-Press-Releases/ESMO-2012-Press-Releases/Equal-access-to-cancer-care-is-a-medical-and-ethical-imperativeGoogle Scholar and we wish that choral collaboration and dialogue between regulators, payers, governments, patient stakeholders and industry will help to ensure that in all countries in EU, high quality cost-effective medical oncology care will include compounds such as the newcomer entrectinib.