Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19
Chung‐Guei Huang, Kuo-Ming Lee, Mei-Jen Hsiao, Shu-Li Yang, Peng-Nien Huang, Yu‐Nong Gong, Tzu-Hsuan Hsieh, Po-Wei Huang, Ya-Jhu Lin, Yi‐Chun Liu, Kuo‐Chien Tsao, Shin‐Ru Shih
Abstract
genes, respectively. We further examined the correlation of genome copy number and virus isolation in different regions of the viral genome, demonstrating that culturable specimens are characterized by high copy numbers with a linear correlation observed between copy numbers of amplicons targeting structural and nonstructural regions. Overall, these results indicate that in addition to the copy number, the integrity of the viral genome should be considered when evaluating the infectivity of clinical SARS-CoV-2 specimens.
Topics & Concepts
VirologyInfectivitySputumVirusCoronavirusViral cultureSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Reverse transcription polymerase chain reactionBiologyVirus isolationCoronavirus disease 2019 (COVID-19)RNA extractionReal-time polymerase chain reactionMicrobiologyRNAMedicineInfectious disease (medical specialty)DiseaseGenePathologyTuberculosisMessenger RNABiochemistrySARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiology