Identification of the peptide epimerase MslH responsible for <scp>d</scp>-amino acid introduction at the C-terminus of ribosomal peptides
Zhi Feng, Yasushi Ogasawara, Tohru Dairi
Abstract
center adjacent to a carboxylic acid in a cofactor-independent manner. We also demonstrate that MslH exhibits broad substrate specificity toward the N-terminal region of the core peptide, showing that MslH-type epimerases offer opportunities in peptide bioengineering.
Topics & Concepts
PeptideEpimerRibosomal RNAPeptide BiosynthesisBiochemistryChemistryRibosomal proteinAmino acidCofactorTryptophanBiosynthesisStereochemistryEnzymeRibosomeGeneRNAChemical Synthesis and AnalysisRNA and protein synthesis mechanismsEnzyme Structure and Function