A ratiometric fluorescent probe for monitoring trace hydrogen peroxide in food and living cell mitochondria
Haotian Xin, Shixian Cao, Guiyi Yang, Yan Huang, Yan Zhang, Kang‐Nan Wang, Duxia Cao
Abstract
Hydrogen peroxide (H 2 O 2 ) has a very important function in the environment and organisms. In this work, a new H 2 O 2 response fluorescent probe OPY-AE based on cyclohexyl-alkenylene-malononitrile structure unit with methylphenylborate as sensing group, was developed. Based on its structural characteristics, OPY-AE can realize color development and ratiometric fluorescence response to H 2 O 2 with good photostability, which enables the probe to achieve the desired level of accuracy in detecting H 2 O 2 . OPY-AE exhibits a relatively fast response rate (9 min) to H 2 O 2 , with a low detection limit of 0.11 μM, so it can be further applied to detect H 2 O 2 in food environments and cell mitochondria with high sensitivity and strong anti-interference ability. The residual H 2 O 2 in food can be quantitatively detected visually in situ through color change and fluorescence ratiometric change of OPY-AE . At the same time, OPY-AE is combined with a detection device as a low-cost and convenient detection tool for fast, visual and quantitative detection of H 2 O 2 in the environment. In addition, OPY-AE is used to realize in-situ tracking and imaging H 2 O 2 in A549 and HeLa cells mitochondria with high contrast and high sensitivity.