High specific immobilization of His-tagged recombinant Microbacterium esterase by Ni-NTA magnetic chitosan microspheres for efficient synthesis of key chiral intermediate of d-biotin
Song He, Xiaomei Wu, Bao-Di Ma, Yi Xu
Topics & Concepts
ChemistryEsteraseChitosanEpichlorohydrinImmobilized enzymeChelationEthylenediamineChromatographyNuclear chemistryCombinatorial chemistryEnzymeBiochemistryOrganic chemistryBiotin and Related StudiesEnzyme Catalysis and ImmobilizationClick Chemistry and Applications