Comparative Anticancer Potentials of Taxifolin and Quercetin Methylated Derivatives against HCT-116 Cell Lines: Effects of <i>O</i>-Methylation on Taxifolin and Quercetin as Preliminary Natural Leads
Hamdoon A. Mohammed, Suliman A. Almahmoud, El‐Sayed M. El‐Ghaly, Firdos Alam Khan, Abdul‐Hamid Emwas, Mariusz Jaremko, Fatimah Almulhim, Riaz A. Khan, Ehab A. Ragab
Abstract
. The viability of the HEK-293 normal cells was found to be significantly higher than the viability of the cancerous cells at all of the tested concentrations, thus suggesting that all the compounds have better inhibitory activity on the cancer cell line. Apoptotic features such as chromatin condensation and nuclear shrinkage were also induced by the compounds. The expression of caspase-3 and caspase-9 genes increased in HCT-116 cell lines after 48 h of treatment, suggesting cell death by the apoptotic pathways. The molecular docking studies showed favorable binding affinity against different pro- and antiapoptotic proteins by these compounds. The docking scores were minimum as compared to the caspase-9, caspase-3, Bcl-xl, and JAK2.