Correction: MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity
Meenalakshmi Chinnam, Chao Xu, Rati Lama, Xiaojing Zhang, Carlos D. Cedeno, Yanqing Wang, Aimee Stablewski, David W. Goodrich, Xinjiang Wang
Abstract
Fig 3. p53-null Mdm2 la/la MEFs and sarcoma cells have defects in and increased and G2-M transition hyperploidy. (A) Cell cycle profiles of Mdm2 la/la : TP53 R/R and Mdm2 +/+ : TP53 R/R MEFs (passage 6) by flow cytometry. Dip, diploid, An, aneuploid. (B) Increased phospho-Histone 3 at Serine 10 (pH3(S10) in p53-deficient Mdm2 la/la sarcoma tissues. Representative histochemical staining of pH3(S10) (a, b) and Ki67 (c, d) in sarcoma tissues from p53 -/-: Mdm2 +/+ (a, c) or p53 -/-: Mdm2 la/la (b, d) mice. Left images at 10x magnification and at 40x magnification of image areas in frame shown on the right. (C) Quantitative analysis of pH3(S10) staining in two p53 -/-: Mdm2 +/+ and three p53 -/-: Mdm2 la/la sarcoma samples. , t test, p = 0.0106. (D) Quantitative analysis of Ki67-positive cells in two p53 -/-: Mdm2 +/+ and three p53 -/-: Mdm2 la/la sarcoma samples. ns, t test, p = 0.604. (E) Mdm2 +/+ -tetp53 and Mdm2 la/la -tetp53 MEFs were used for BrdU labeling experiments. Gating settings are shown to define viable, singlet and BrdU-positive cells. (F) Diploid S (Dip S) and hyperploid S (Hyp S) fractions of Mdm2 +/+ -tetp53 and Mdm2 la/la -tetp53 MEFs were presented. (G)