Bionic biphasic composite scaffolds with osteochondrogenic factors for regeneration of full-thickness osteochondral defects
Yanbo Zhang, Yu Han, Yachen Peng, Jie Lei, Fei Chang
Abstract
-glycolide)/hydroxyapatite porous scaffold was immobilized with bone morphogenetic protein-2 as the bottom layer to facilitate subchondral repair. The intermediate interface, being a combination of the two layers, was expected to reconstruct the calcification zone. Gross evaluation, micro-computed tomography, and magnetic resonance imaging indicated that at 6 months after implantation, the scaffold encapsulating mesenchymal stem cells and osteochondrogenic factors had a better effect compared with other groups. More importantly, the composition of the new cartilage and bone was confirmed by western blotting, H&E staining, immunohistochemistry, and immunofluorescence. Taken together, this bionic composite scaffold with osteochondrogenic factors offers a promising option for the repair of full-thickness osteochondral defects.