GREEN SYNTHESIS OF SILVER AND COPPER NANOPARTICLES FROM LEAVES OF EUCALYPTUS GLOBULUS AND ASSESSMENT OF ITS ANTIBACTERIAL POTENTIAL TOWARDS XANTHOMONAS CITRI PV. CITRI CAUSING CITRUS CANKER
Muhammad Atiq Muhammad Atiq, Hafiz M. R. Mazhar, Neetu Rajput, Usama Ahmad, Akhtar Hameed, Abdul Mubeen Lodhi, Muhammad Usman, A. NAWAZ, Muhammad Ammar, M. KHALID
Abstract
Green synthesis of nanoparticles has ushered in a new research field known as green or phytonanotechnology. Plant based nanotechnology has emerged as an imperative tool for the management of plant diseases. In comparison to other conventional techniques, green synthesis of various nanoparticles was found to be non-toxic, low-cost, and profitable, resulting in more stable synthesized materials. Plant-extract based methods for synthesizing nanoparticles are far more efficient and cost-effective to manage plant diseases. For the management of citrus canker, silver and copper nanoparticles were evaluated under lab conditions. The highest inhibition zone was produced under silver + copper nanoparticles (21.06 mm) follow by silver (18.26 mm) and copper nanoparticles (15.27 mm) respectively. In field, maximum disease incidence was exhibited by copper nanoparticles (41.24%), and minimum disease incidence (35.23%) was observed when silver and copper nanoparticles were used in combination as compared to the control. Minimum disease severity (23.23%) was observed when of silver and copper nanoparticles were applied in combination followed by silver nanoparticles (28.31%) and copper nanoparticles (35.23%) as compared to control under field conditions. Complete Randomized Design (CRD) was used for the laboratory and greenhouse experiments while Randomized Complete Block Design (RCBD) was used for the field experiment.