Multiplex base editing to convert TAG into TAA codons in the human genome
Yuting Chen, Eriona Hysolli, Anlu Chen, Stephen Casper, Songlei Liu, Yi Yang, Chenli Liu, George M. Church
Abstract
Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells.
Topics & Concepts
Genome editingGenomeMultiplexComputational biologyGeneStop codonHuman genomeBiologyBase (topology)GeneticsComputer scienceMathematicsMathematical analysisCRISPR and Genetic EngineeringVirus-based gene therapy researchRNA and protein synthesis mechanisms