A Nuclear Export Signal Is Required for cGAS to Sense Cytosolic DNA
Hong Sun, Yu Huang, Shan Mei, Fengwen Xu, Xiaoman Liu, Fei Zhao, Lijuan Yin, Di Zhang, Wei Liang, Chao Wu, Shichao Ma, Jianwei Wang, Shan Cen, Chen Liang, Siqi Hu, Fei Guo
Abstract
. Mutating this NES leads to the sequestration of cGAS within the nucleus and the loss of interferon response to cytosolic DNA treatment, and it further determines the key amino acid to L172. Collectively, our data demonstrate that the cytosolic DNA-sensing function of cGAS depends on its presence within the cytoplasm, which is warranted by a functional NES.
Topics & Concepts
Nuclear export signalCytoplasmCytosolBiologyCell biologyNuclear localization sequenceNuclear transportDNASubcellular localizationNucleusCell nucleusBiochemistryEnzymeinterferon and immune responsesImmune Response and InflammationRNA Research and Splicing