Ginsenoside Re Treatment Attenuates Myocardial Hypoxia/Reoxygenation Injury by Inhibiting HIF-1α Ubiquitination
Huiyuan Sun, Shukuan Ling, Dingsheng Zhao, Jianwei Li, Yang Li, Hua Qu, Ruikai Du, Ying Zhang, Feng Xu, Yuheng Li, Caizhi Liu, Guohui Zhong, Shuai Liang, Zizhong Liu, Xingcheng Gao, Xiaoyan Jin, Yingxian Li, Dazhuo Shi
Abstract
mRNA, while protein level of HIF-1α increased and that of HIF-1α[Ub]n decreased following ginsenoside Re treatment. Immunoprecipitation results showed that the amount of HIF-1α bound to VHL substantially decreased following ginsenoside Re treatment. In addition, ginsenoside Re treatment increased the expression of GLUT1 (glucose transporter 1) and REDD1 (regulated in development and DNA damage response 1), which are targets of HIF-1α and are critical for cell metabolism and viability. These results suggested that Ginsenoside Re treatment attenuated the myocardial injury induced by H/R, and the possible mechanism was associated with the inhibition of HIF-1α ubiquitination.