Discovery of sandwich type COVID-19 nucleocapsid protein DNA aptamers
Liyun Zhang, Xiaona Fang, Xingbo Liu, Huichao Ou, Haiyan Zhang, Jin‐Jun Wang, Qian Li, Huanyi Cheng, Wenyi Zhang, Zhaofeng Luo
Abstract
value of 0.49 nM. Interestingly, we found that the four pairs of aptamers could bind to Np successively, suggesting a sandwich-type interaction. Using these sandwiched aptamers in ELISA and colloidal gold immunochromatographic strips, we were able to detect Np at the tens of pM level. The results demonstrate that aptamers are powerful molecular tools for virus detection, diagnosis, and antiviral therapy.
Topics & Concepts
AptamerCoronavirus disease 2019 (COVID-19)DNASevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Virology2019-20 coronavirus outbreakComputational biologyMolecular biologyBiologyChemistryGeneticsMedicineOutbreakInfectious disease (medical specialty)DiseasePathologyAdvanced biosensing and bioanalysis techniquesSARS-CoV-2 detection and testingBiosensors and Analytical Detection